Antibiotic Resistance as a Virulence Factor of Opportunistic Microorganisms

Introduction: A large number of infectious processes are associated with opportunistic microorganisms. The phenotype antibiotic resistance such pathogens is multidrug-resistant strains the presence various β-lactamases. Our objective was to determine phenotypic and genotypic features staphylococci, enterobacteria, non-fermenting Gram-negative bacteria – cause diseases in patients health facilities Nizhny Novgorod. Material methods: Using classical microbiological methods molecular genetic studies, we analyzed 486 microorganisms isolated from upper respiratory tract, intestines, urine, wound discharge 2019–2020. In all isolates, determined by disco-diffusion method (Bioanalyse, Turkey) using Multiscan FC spectrophotometer (ThermoScientific, Finland) Microlatest tablets (PLIVA-Lachema, Czech Republic), along mechanisms PCR on CFX96 device (BioRad, USA) AmpliSens kits (Russia). Results discussion: results showed that most prevalent causative agents (40.7 %) were bacteria, which Enterobacteriaceae accounted for 27.1 % 13.6 cases, respectively. Staphylococci 37.6 patients: S. aureus coagulase-negative staphylococci induced 13.4 24.2 analysis isolates a high level antimicrobial hospitals, regardless isolation locus. methicillin-resistant found 26.3 37.9 respectively; mecA gene 89.0 staphylococci. highest antibiotic-resistant among observed K. pneumoniae, A. baumannii, P. aeruginosa. We established 61.7 75.1 58.2 aeruginosa resistant carbapenems. studies confirmed serine carbapenemases KPC OXA groups pneumoniae baumannii; genes metallo-β-lactamase VIM group 40.9 production numerous β-lactamases determinants genome virulent properties Conclusion: developing chronic process. Today, wide spread serious public problem, determines need constant monitoring identify potent antibiotics ways eradication strains.